Here we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare ...
The ability to turn a gene off only in a specific cell type is essential to modern life science. Thanks to the Cre-Controlled CRISPR it has just became simpler. The new method developed by researchers ...