Cre-loxP, a powerful tool for preclinical cancer research, possesses intrinsic pitfalls that can alter result accuracy, according to an editorial paper in Oncoscience. Cancer and other diseases could ...

Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA sequence loxP, which is its target. This vector allows the creation of ...

A new editorial paper has been published in Oncoscience, titled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this editorial, researchers Piotr Czarnota and ...

To further improve this promoter, we evaluated the length of the upstream region of the SCG10 promoter. Luc constructs driven by 2 and 3.7 kb SCG10 gene promoter (S2 and S3.7), both of which contained ...

Heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to ...

Despite the major impact of Cre-loxP technology on biomedical research, numerous studies have demonstrated the need for caution in its use. The main problem is that often, the Cre activity level is ...

Chances are if you use mice in your research, you are intimately acquainted with the Cre/LoxP recombination system. (For the uninitiated out there, this is a system to achieve tissue- or cell ...

In traditional knockout studies, the gene is deleted altogether. However, deleting genes that are essential to embryonic development is lethal. Conditional gene knockout can be activated in an adult ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...

“One limitation [of Cre-LoxP], the focus of this editorial, is the potential loss of fidelity of Cre recombinase expression especially in the context of modeling cancer in mice.” “It is based on ...

Mice and newts were applied to the one-step Cre-loxP organism creation technology by TAx9. In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short DNA ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...